Show simple item record

dc.contributor.authorCasabianca, S. (Silvia)
dc.contributor.authorCasabianca, A. (Anna)
dc.contributor.authorRiobó, P. (Pilar) 
dc.contributor.authorFranco, J.M. (José Mariano) 
dc.contributor.authorVila, M. (Magda)
dc.contributor.authorPenna, A. (Antonella)
dc.date.accessioned2015-11-20T09:15:28Z
dc.date.issued2013-03-12
dc.identifier.issn0013-936X*
dc.identifier.urihttp://hdl.handle.net/10508/9894
dc.description.abstractWe report the development and validation of a qPCR based method for estimation of the toxic benthic dinoflagellate Ostreopsis cf. ovata in the complex matrix of marine aerosol at Sant Andreu de Llavaneres beach (northwestern Mediterranean Sea). Toxic events in humans after inhalation or cutaneous contact have been reported during O. cf. ovata blooms and were attributed to palytoxin (PLTX)-like compounds produced by this microalga. Similar PCR efficiencies of plasmid and cellular environmental standard curves (98 and 100%, respectively) allowed obtaining the rDNA copy number per cell. The analytical sensitivity was set at 2 × 100 rDNA copy number and 8 × 10–4 cell per reaction. Based on spiking experiments, we evaluated the aerosol filter inhibitory activity and recovery rate of cells from filters, then normalized the abundance data of toxic O. cf. ovata. The abundance in marine aerosol during the bloom varied in the range of 1–102 cells per filter. Analytical determinations were also applied to detect palytoxin in field samples. No palytoxin was detected in the aerosol filters, and the estimation of PLTX like-compound concentrations in microepiphytic assemblages varied between 0.1 and 1.2 pg/cell.es_ES
dc.language.isoenges_ES
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.titleQuantification of the Toxic Dinoflagellate Ostreopsis spp. by qPCR Assay in Marine Aerosoles_ES
dc.typearticlees_ES
dc.identifier.bibliographicCitationEnvironmental Science & Technology, 47. 2013: 3788-3795*
dc.description.versionVersión del editores_ES
dc.date.embargoEndDateindefinidoes_ES
dc.publisher.centreCentro Oceanográfico de Vigoes_ES
dc.rights.accessRightsembargoedAccesses_ES
dc.description.impact5,2280*
dc.identifier.doi10.1021/es305018ses_ES
dc.coverage.spatialStudyMediterranean Seaen_US
dc.coverage.spatialStudyWestern Mediterranean Seaen_US
dc.coverage.spatialStudyGulf of Lionsen_US


Files in this item

    Show simple item record

    Attribution-NonCommercial-NoDerivs 3.0 Spain
    Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Spain