Yessotoxins production during the culture of Protoceratium reticulatum strains isolated from Galician Rias Baixas (NW Spain)
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http://www.sciencedirect.com/science/article/pii/S1568988312001515Date
2013-01Type
research articleKeywords
Culture growthLC–MS3 analysis
Protoceratium reticulatum
Yessotoxins
Abstract
Yessotoxins (YTXs) production along the culture growth of three strains of the dinoflagellate Protoceratium reticulatum isolated from seawater of Galician Rias Baixas, Spain was investigated. Quantification and toxin profile determination in both cells and culture medium along the growth curve were performed by liquid chromatography–mass spectrometry (LC–MS3) analysis. The YTX profile was very similar among strains, the three algal strains produce mainly YTX and also some YTX analogs. Among the strains the maximum toxin production ranged between 416 and 576 ng mL−1. This is the first report about YTX production by P. reticulatum isolated in Galician coast, NW Spain.
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