Identification and Quantification of the Age-pigment, Lipofuscin, in Brains of the Deep-water Rose Shrimp Aristeus antennatus (Risso, 1816)
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Aristeus antennatus is a demersal penaeid mainly living at depth of 400-800 m in Mediterranean Sea. Since this specie is a high economic important fishery of the Spanish Mediterranean waters, the studies of age structure of the population are essential for an efficient assessment of the resource. Exist a inability to accurately estimate the age through conventional methods in crustacean and a new methodology have been developed on basis of the gradual deposition of lipofuscin in post-mitotic tissues for the ageing of animals. The identification of the age-pigment have been realized on transversal sections of the olfactory lobe cells mass (OLCM) in brain of wild-caught A. antennatus, observed under epifluorescence microscopy and quantified using image analysis techniques. Three different measurements of lipofuscin levels (% area fraction, granule density and granule mean size) were recorded in ten distinet sections of OLCM by individuals, body size class and sex. Relationship between body ...
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