Characterization of the interferon pathway in the teleost fish gonad against the vertically transmitted viral nervous necrosis virus
MetadataShow full item record
AuthorsValero-Cuesta, Y. (Yulema); Morcillo, P. (Patricia); Meseguer, J. (José); Buonocore, F. (Francesco); Esteban, M.A. (María Ángeles); Chaves-Pozo, E. (Elena); Cuesta, A. (Alberto)
KeywordsNodavirus (VNNV); interferon (IFN) pathway; gonad; gilthead seabream; European sea bass
One of the most powerful innate immune responses against viruses is mediated by type I IFN. In teleost fish, it is known that virus infection triggers the expression of ifn and many IFN-stimulated genes, but the viral RNA sensors and mediators leading to IFN production are scarcely known. Thus, we have searched for the presence of these genes in gilt-head sea bream (Sparus aurata) and European sea bass (Dicentrarchus labrax), and evaluated their expression after infection with viral nervous necrosis virus (VNNV) in the brain, the main viral target tissue, and the gonad, used to transmit the virus vertically. In sea bream, a fish species resistant to the VNNV strain used, we found an upregulation of the genes encoding MDA5 (melanoma differentiation-associated gene 5), TBK1 (TANK-binding kinase 1), IRF3 (IFN regulatory factor 3), IFN, Mx [myxovirus (influenza) resistance protein] and PKR (dsRNA-dependent protein kinase receptor) proteins in the brain, which were unaltered in the gonad and ...
The following license files are associated with this item: