Reproduction of cultured Senegalese sole (Solea senegalensis): Protocol for hormonal inducted ovulation, stripping of eggs and artificial fertilization
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AuthorsRasines, I. (Inmaculada); Gómez, M.; Martín, I.; Rodríguez, C.; Gutiérrez, J.R.; Calvo, B.; Mañanós-Sánchez, E. (Evaristo); Chereguini-Fernández-Maquieira, M.O. (María del Olvido)
The Senegalese sole, Solea senegalensis (Kaup, 1858), is one of the leading candidates for the diversification of the Southern European pisciculture industry. However, there are still several problems that restrict the consolidation of this species at an industrial level. One of the primary problems associated with culturing Senegalese sole is the extent of reproductive dysfunctions detected in individuals hatched and reared in captivity (generation F1). Spontaneous spawns are scarce and generally not fertilized. So far, it has not been able to close the farming cycle nor draw genetic improvement plans. The aim of this work was to set a protocol for an hormonal inducted ovulation with synthetic analogs of gonadotropin releasing hormone (GnRHa) and the artificial fertilization of eggs obtained by stripping. Females in stage III of ovarian development according to the classification of Anguis and Cañavate (2005) were selected for all experiments. First, the most suitable method of ...
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