Fish granulocytes express a constitutively active androgen receptor variant
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AuthorsSánchez-Hernández, M. (Miriam); Arizcun-Arizcun, M. (Marta); García-Alcázar, A. (Alicia); Sarropoulou, E. (Elena); Mulero, V. (Victoriano); García-Ayala, A. (Alfonsa)
In humans, alternative splicing of androgen receptor (AR) is usually involved in some diseases. However, our knowledge about the presence of AR variants in other species and its importance for immunity is scant. Here, we report the identification of a constitutively active AR variant lacking the ligand-binding domain (LBD), ARDLBD, in the fish gilthead seabream. ARDLBD is expressed in the testis and the headkidney (HK), and its expression varies with the reproductive stage and is correlated with plasma testosterone (T). In addition, ARDLBD is expressed in acidophilic granulocytes (AGs), which are the functional equivalent of mammalian neutrophils, but not in macrophages, and its expression is modulated by both T and immune stimuli. Notably, AR and ARDLBD were able to interact, being the activity of AR dominant at all concentrations tested of the ligand. These results reveal a new mechanism for the regulation of neutrophil biology in vertebrates and explain the conflicting results ...
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