A Microsatellite Genetic Map of the Turbot (Scophthalmus maximus)
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AuthorsBouza, C. (Carmen); Hermida, M. (Miguel); Pardo, B. (Belén); Fernández, C. (Carlos); Fortes, G. (Gloria); Castro, J. (Jaime); Sánchez, L. (Laura); Presa, P. (Pablo); Pérez, M. (Montse); Sanjuán, A. (Andrés); Carlos, A. (Alejandro) de; Álvarez-Dios, J.A. (José Antonio); Ezcurra, S. (Susana); Cal, R. (Rosa); Piferrer, F. (Francesc); Martínez, P. (Paulino)
A consensus microsatellite-based linkage map of the turbot (Scophthalmus maximus) was constructed from two unrelated families. The mapping panel was derived from a gynogenetic family of 96 haploid embryos and a biparental diploid family of 85 full-sib progeny with known linkage phase. A total of 242 microsatellites were mapped in 26 linkage groups, six markers remaining unlinked. The consensus map length was 1343.2 cM, with an average distance between markers of 6.5 6 0.5 cM. Similar length of female and male maps was evidenced. However, the mean recombination at common intervals throughout the genome revealed significant differences between sexes, 1.6 times higher in the female than in the male. The comparison of turbot microsatellite flanking sequences against the Tetraodon nigroviridis genome revealed 55 significant matches, with a mean length of 102 bp and high sequence similarity (81–100%). The comparative mapping revealed significant syntenic regions among fish species. ...
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