Identification and functional characterization of a new IL-I family member, IL-IFm2, in most evolutionarily advanced fish
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AuthorsAngosto, D.; Montero, J.; López-Muñóz, A. (Azucena); Alcaraz-Pérez, F.; Bird, S.; Sarropoulou, E. (Elena); Abellán-Martínez, E. (Emilia); Meseguer, J. (José); Sepulcre, M.P. (Maria Pilar); Mulero, V. (Victoriano)
The IL-1 family consists of 11 members that play an important role as key mediators in inflammation and immunity. Here, we report the identification of a new member of the IL-1 family (IL-1Fm2) that is present in species belonging to the most evolutionarily advanced group of teleost fish (Series Percomorpha), including Perciformes, Beloniformes, Gasterosteiformes, Cyprinodontiformes and Pleuronectiformes. However, IL-1Fm2 seems to be absent in Tetraodontiformes, which also belong to the Percomorpha. The expression pattern of gilthead seabream IL-1Fm2 revealed that although it was hardly induced by PAMPs, the combination of PAMPs and recombinant IL-1Fm2 synergistically induced its expression in macrophages and granulocytes. In addition, recombinant IL-1Fm2 was able to activate the respiratory burst of seabream phagocytes and to synergistically induce the expression of IL-1b, TNF-a, IL-8 and IL-10 when combined with PAMPs. Finally, although gilthead seabream IL-1Fm2 did not show a conserved ...