Estrogen Signaling through the G Protein-Coupled Estrogen Receptor Regulates Granulocyte Activation in Fish
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AuthorsCabas, I. (Isabel); Rodríguez-Cabello, C. (Cristina); Abellán-Martínez, E. (Emilia); Meseguer, J. (José); Mulero, V. (Victoriano); García-Ayala, A. (Alfonsa)
Neutrophils are major participants in innate host responses. It is well known that estrogens have an immune-modulatory role, and some evidence exists that neutrophil physiology can be altered by these molecules. Traditionally, estrogens act via classical nuclear estrogen receptors, but the identification of a G protein–coupled estrogen receptor (GPER), a membrane estrogen receptor that binds estradiol and other estrogens, has opened up the possibility of exploring additional estrogen-mediated effects. However, information on the importance of GPER for immunity, especially, in neutrophils is scant. In this study, we report that gilthead seabream (Sparus aurata L.) acidophilic granulocytes, which are the functional equivalent of mammalian neutrophils, express GPER at both mRNA and protein levels. By using a GPER selective agonist, G1, it was found that GPER activation in vitro slightly reduced the respiratory burst of acidophilic granulocytes and drastically altered the expression ...