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dc.contributor.authorBerkovich, N.es_ES
dc.contributor.authorCorriero, A.es_ES
dc.contributor.authorSantamaria, N.es_ES
dc.contributor.authorMylonas, C.C.es_ES
dc.contributor.authorVassallo-Agius, R.es_ES
dc.contributor.authorde-la-Gándara, F. (Fernando) es_ES
dc.contributor.authorMeiri-Ashkenazi, I.es_ES
dc.contributor.authorZlatnikov, V.es_ES
dc.contributor.authorGordin, H.es_ES
dc.contributor.authorBridges, C.R.es_ES
dc.contributor.authorRosenfeld, H.es_ES
dc.date.accessioned2014-11-21T10:28:18Z
dc.date.available2014-11-21T10:28:18Z
dc.date.issued2013es_ES
dc.identifier.issn0016-6480*
dc.identifier.urihttp://hdl.handle.net/10508/8615
dc.description.abstractAs part of the endeavor aiming at the domestication of Atlantic bluefin tuna (BFT; Thunnus thynnus), first sexual maturity in captivity was studied by documenting its occurrence and by characterizing the key hormones of the reproductive axis: follicle stimulating hormone (FSH) and luteinizing hormone (LH). The full length sequence encoding for the related hormone b-subunits, bftFSHb and bftLHb, were determined, revealing two bftFSHb mRNA variants, differing in their 50 untranslated region. A quantitative immuno-dot-blot assay to measure pituitary FSH content in BFT was developed and validated enabling, for the first time in this species, data sets for both LH and FSH to be compared. The expression and accumulation patterns of LH in the pituitary showed a steady increase of this hormone, concomitant with fish age, reaching higher levels in adult females compared to males of the same age class. Conversely, the pituitary FSH levels were elevated only in 2Y and adult fish. The pituitary FSH to LH ratio was consistently higher (>1) in immature than in maturing or pubertal fish, resembling the situation in mammals. Nevertheless, the results suggest that a rise in the LH storage level above a minimum threshold may be an indicator of the onset of puberty in BFT females. The higher pituitary LH levels in adult females over males may further support this notion. In contrast three year-old (3Y) males were pubertal while cognate females were still immature. However, it is not yet clear whether the advanced puberty in the 3Y males was a general feature typifying wild BFT populations or was induced by the culture conditions. Future studies testing the effects of captivity and hormonal treatments on precocious maturity may allow for improved handling of this species in a controlled environment which would lead to more cost-efficient farming.es_ES
dc.language.isoenges_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Españaes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/es_ES
dc.subjectAtlantic bluefin tunaes_ES
dc.subjectDot-blot-immunoassayes_ES
dc.subjectGonadotropinses_ES
dc.subjectPubertyes_ES
dc.subjectSexual dimorphismes_ES
dc.subjectFish reproductiones_ES
dc.subjecttunaes_ES
dc.titleIntra-pituitary relationship of follicle stimulating hormone and luteinizing hormone during pubertal development in Atlantic Bluefin tuna (Thunnus thynnus)es_ES
dc.typearticlees_ES
dc.identifier.bibliographicCitationGeneral and Comparative Endocrinology, 194. 2013: 10-23es_ES
dc.description.versionVersión del editores_ES
dc.date.embargoEndDateindefinidoes_ES
dc.publisher.centreCentro Oceanográfico de Murciaes_ES
dc.relation.projectIDSELFDOTTes_ES
dc.rights.accessRightsembargoedAccesses_ES
dc.description.impact3es_ES
dc.identifier.doi10.1016/j.ygcen.2013.08.005es_ES


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    Atribución-NoComercial-SinDerivadas 3.0 España
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