Induction of triploidy in the turbot (Scophthalmus maximus): I. Ploidy determination and the effects of cold shocks
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AuthorsPiferrer, F. (Francesc); Cal, R. (Rosa); Álvarez-Blázquez, B. (Blanca); Sánchez, L. (Laura); Martínez, P. (Paulino)
The basis for induction of triploidy in the turbot by applying cold shocks shortly after fertilization (AF) was studied. Since this species exhibits a polymorphism in a number of nucleolar organizing regions (NOR), determination of the ploidy level through NOR analysis was first validated. Results showed that NOR analysis could discriminate well between diploid and triploid individuals whose ploidy level was verified karyotypically (n=44 chromosomes in diploids; n=66 in triploids). In diploids, the mean number of NOR per cell ranged from 1.10 to 1.85, whereas in triploids, it ranged from 1.50 to 2.35. However, histogram distribution of data on mean number of NOR per cell showed that the number of fish in the overlapping region (NOR number between 1.50 and 1.85) was very low. Cold-shocked fish with a NOR value >1.735 were considered triploids. The error in ploidy assessment using NOR analysis in the turbot was found to be consistently around 3% and always <5%. In this way, NOR analysis ...
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