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dc.contributor.authorFlynn, K.J. (Kevin)es_ES
dc.contributor.authorFlynn, K. (Krystyna)es_ES
dc.contributor.authorJohn, E.H. (Eurgain)es_ES
dc.contributor.authorReguera, B. (Beatriz) es_ES
dc.contributor.authorReyero, M.I. (Isabel)es_ES
dc.contributor.authorFranco, J.M. (José Mariano) es_ES
dc.date.accessioned2014-11-21T09:56:18Z
dc.date.available2014-11-21T09:56:18Z
dc.date.issued1996es_ES
dc.identifier.urihttp://hdl.handle.net/10508/6830
dc.description.abstractThe paralytic shellfish poison prducing dinoflagellate Gymnodiniun catemrum was subjected to changes in salinity, phosphate, ammonium and nitrate using continuous culture and batch culture methods. In contrast with other algae, this species showed very slow changes in the concentration of intracellular amino acids, in the Gln:Glu ratio, and, in contrast with Alrsandnum spp., only slow changes in toxin content, during such events as N-feeding of Ndeprived cells or during nutrient deprivation. This organism was found to be very susceptible to disturbance; maximum growth rates around 0.25–0.3 day−1 with a minimum C:N mass ratio of 5.5, were attained when cultures were only disturbed by sampling once a day. P-deprived cells were larger (twice the usual C content of 4 ng C cell−1 and volume of 20 pl). The content of free amino acids was always low (5% of cell-N), with low contributions made by arginine (the precursor for paralytic shellfish toxins). Cells growing using ammonium had the lowest C:N ratios and the highest proportion of intracellular amino acids as arginine. The toxin profile (equal mole ratios of dcSTX, GTX5, dcGT2/3 C1 and C2, and half those values for C3 and C4) was stable and the toxin concentration varied between 0.2 and 1 mM STX equivalents (highest when ammonium was not limiting, lowest in P-deprived cells, though as the latter were larger toxin per cell was not so variable). Decreased salinity did not result in increases in toxin content. Significant amounts of amino acids (mainly serine and glycine, with a total often exceeding 4 μM) accumulated in the growth medium during batch growth even though the cultures were not bacteria free.
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Españaes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/es_ES
dc.titleChanges in toxins, intracellular and dissolved free aminoacids of the toxic dinoflagellate Gymnodinium catenatum in response to changes in inorganic nutrients and salinityes_ES
dc.typeresearch articlees_ES
dc.identifier.bibliographicCitationJournal of Plankton Research, 18(11). 1996: 2093-2111es_ES
dc.relation.publisherversionhttp://plankt.oxfordjournals.org/content/18/11/2093.abstract
dc.publisher.centreCentro Oceanográfico de Vigoes_ES
dc.rights.accessRightsopen accesses_ES
dc.description.impact2es_ES
dc.identifier.doi10.1093/plankt/18.11.2093


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    Atribución-NoComercial-SinDerivadas 3.0 España
    Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España