Rapid flow cytometry method for triploidy determination in turbot (Scopthalmus maximus L.)

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URI: http://hdl.handle.net/10508/529
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Authors
Vázquez, E. (Emilia); Fernández-Pato, C. (Carlos); Martínez-Tapia, C. (Concha); Blanco, G. (Gloria); Sánchez Prado, J.A. (José Antonio)
Date
2012-03-21
Type
other
Keywords
flow cytometry
larvae
triploidy
turbot
Abstract
Accurate methods for assessing triploidy are important because treatments designed to induce poliploidy are not 100% and offspring from treated eggs are usually a mixture of triploids and diploids.
 
Flow cytometry was more recently applied to triploidy determination and usually carried out using erythrocytes.
 
This paper describes a rapid and simple method for triploidy determination from the larval stage of turbot (Scopthalmus maximus) by flow cytometry.
 
High-quality histograms were obtained from turbot larvae and the very good reproductibility provided unambigous discrimination of diploids and triploids even in early stages development.