Rapid flow cytometry method for triploidy determination in turbot (Scopthalmus maximus L.)
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AuthorsVázquez, E. (Emilia); Fernández-Pato, C. (Carlos); Martínez-Tapia, C. (Concha); Blanco, G. (Gloria); Sánchez Prado, J.A. (José Antonio)
Accurate methods for assessing triploidy are important because treatments designed to induce poliploidy are not 100% and offspring from treated eggs are usually a mixture of triploids and diploids.Flow cytometry was more recently applied to triploidy determination and usually carried out using erythrocytes.This paper describes a rapid and simple method for triploidy determination from the larval stage of turbot (Scopthalmus maximus) by flow cytometry.High-quality histograms were obtained from turbot larvae and the very good reproductibility provided unambigous discrimination of diploids and triploids even in early stages development.