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dc.contributor.authorMontero, J. (Jana)
dc.contributor.authorGómez-Casado, E. (Eduardo)
dc.contributor.authorGarcía-Alcázar, A. (Alicia) 
dc.contributor.authorMeseguer, J. (José)
dc.contributor.authorMulero, V. (Victoriano)
dc.date.accessioned2014-08-20T06:31:24Z
dc.date.available2014-08-20T06:31:24Z
dc.date.issued2014-11
dc.identifier.urihttp://hdl.handle.net/10508/2028
dc.description.abstractAdjuvants emerge as the better tool to enhance the efficacy of vaccination. Traditional adjuvants used in aquaculture cause adverse alterations in fish. Thus, it is necessary the development of new adjuvants able to stimulate the immune system and generate high protection against infectious pathogens with minimal undesirable effects. To this end, flagellin emerges as an attractive candidate due to its potency to stimulate the immune response of fish. In the current study, we have evaluated the ability of recombinant flagellin from Marinobacter algicola (MA) and Vibrio vulnificus (Vvul), a non-pathogenic and a pathogenic bacteria, respectively, to stimulate the innate immune system of gilthead seabream (Sparus aurata L.) in comparison with the classical flagellin from Salmonella enterica serovar Thyphimurium (Salmonella Thyphimurium, STF). Intraperitoneal injection of MA and Vvul resulted in a strong inflammatory response characterized by increased reactive oxygen species production and the infiltration of acidophilic granulocytes at the injection site. Interestingly, however, only flagellin from MA consistently induced the expression of the gene encoding pro-inflammatory interleukin-1. These effects were further confirmed in vitro, where a dose-dependent activation of macrophages and acidophilic granulocytes by MA and Vvul flagellins was observed. In contrast, STF flagellin was found to be less potent in either in vivo or in vitro experiments. Our results suggest the potential use of MA and Vvul flagellins as immunostimulants and adjuvants for fish vaccination.es_ES
dc.language.isoenges_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectFlagellines_ES
dc.subjectAdjuvantes_ES
dc.subjectImmunostimulantses_ES
dc.subjectSeabreames_ES
dc.subjectTeleostses_ES
dc.subjectFishes_ES
dc.titleFlagellin from Marinobacter algicola and Vibrio vulnificus activates the innate immune response of gilthead seabreames_ES
dc.typeresearch articlees_ES
dc.identifier.bibliographicCitationDevelopmental & Comparative Immunology, 47 (1). 2014: 160-167*
dc.type.hasVersionAMes_ES
dc.publisher.centreCentro Oceanográfico de Murciaes_ES
dc.rights.accessRightsopen accesses_ES
dc.identifier.doi10.1016/j.dci.2014.07.003es_ES


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    Atribución-NoComercial-SinDerivadas 3.0 España
    Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España