Natural feed after weaning improves the reproductive status of Solea senegalensis breeders
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AuthorsMartín, I.; Riesco, M.F. (Marta); Chaves-Pozo, E. (Elena); Rodríguez-Calvo, C. (Concepción); Martín-Vazquez, J. M.; Robles, V. (Vanesa); Chereguini-Fernández-Maquieira, M.O. (María del Olvido); Rasines, I. (Inmaculada)
The aim of this study was to evaluate the effect of long term natural feeding in the subsequent reproductive status of 4 years old cultured Solea senegalensis, and to determine if the potential changes were structural or feeding dependent. To this aim, two different feeding regimes were used from one year after weaning and during the following 3 years; 1) a commercial dry food diet and 2) a natural feeding regime. After this period, the proportion of fluent males and the evolution of maturity stages of females over a breeding season were studied. A complete sperm quality analysis assessment was carried out, including individual volumes, motility, density and curvilinear, rectilinear and mean velocities of spermatozoa. Moreover, viability and apoptosis indexes were analyzed as indicator of molecular sperm membrane integrity. Additionally, a morphological characterization of the testes during the spawning season was conducted. Finally, both groups were fed with the same commercial pellets ...