European sea bass brain DLB-1 cell line is susceptible to nodavirus: A transcriptomic study
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AuthorsChaves-Pozo, E. (Elena); Bandín, I. (Isabel); Olveira, J.G. (José G); Esteve-Codina, A. (Anna); Gómez-Garrido, J. (Jèssica); Dabad, M. (Marc); Alioto, T. (Tyler); Esteban, M.A. (María Ángeles); Cuesta, A. (Alberto)
European sea bass
DBL-1 cell line
Viral diseases are responsible for high rates of mortality and subsequent economic losses in modern aquaculture. The nervous necrosis virus (NNV) produces viral encephalopathy and retinopathy (VER), which affects the fish central nervous system. It is considered one of the most serious viral diseases in marine aquaculture, the European sea bass (Dicentrarchus labrax) being amongst the most susceptible. We have evaluated the European sea bass brain derived cell line (DLB-1) susceptibility to NNV genotypes and evaluated its transcriptomic profile. DLB-1 cells supported NNV gene transcription and replication since strains belonging to the four NNV genotypes produce cytopathic effects. Afterwards, DLB-1 cells were infected with an RGNNV strain, the one which showed the highest replication, for 12 and 72 h and an RNA-seq analysis was performed to identify potential genes involved in the host-NNV interactions. Differential expression analysis showed the up-regulation of many genes related to ...
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