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dc.contributor.authorBetancor, M.B.
dc.contributor.authorOboh, A.
dc.contributor.authorOrtega-García, A. (Aurelio) 
dc.contributor.authorMourente, G.
dc.contributor.authorNavarro, J.C.
dc.contributor.authorde-la-Gándara, F. (Fernando) 
dc.contributor.authorTocher, D.R.
dc.contributor.authorMonroig, O.
dc.date.accessioned2020-06-22T16:46:06Z
dc.date.available2020-06-22T16:46:06Z
dc.date.issued2019
dc.identifier.issn10964959
dc.identifier.urihttp://hdl.handle.net/10508/11445
dc.description.abstractElongation of very long-chain fatty acid 4 (Elovl4) proteins are involved in the biosynthesis of very long-chain (> C24) fatty acids and in many teleost fish species they are key enzymes in the pathway for the production of docosahexaenoic acid (DHA; 22:6n-3) from eicosapentaenoic acid (EPA; 20:5n-3). Therefore, Elovl4 may be particularly important in Atlantic bluefin tuna (ABT; Thunnus thynnus) characterised by having high DHA to EPA ratios. The present study cloned and characterised both the function and expression of an elovl4 cDNA from ABT. The Elovl4 had an open reading frame of 915 base pairs encoding a putative protein of 304 amino acids. Alignment and phylogenetic analyses indicated that the Elovl4 isoform identified in the present study was an Elovl4b. Functional characterisation demonstrated that the Elovl4b enzyme had elongase activity towards all the polyunsaturated fatty acid (PUFA) substrates assayed. The ABT Elovl4b contributed to DHA biosynthesis by elongation of EPA and DPA to 24:5n-3, the latter being desaturated to 24:6n-3 by the action of fads2 (Δ6 desaturase). Additionally, the ABT Elovl4b has a role in the biosynthesis of very long-chain PUFA up to C34, compounds of key structural roles in neural tissues such as eye and brain, which had high levels of elovl4b transcripts. Surprisingly, while the relative expression of fads2, required for the production of DHA from EPA, was increased in liver of ABT fed a diet with reduced levels of EPA and DHA, expression of elovl4b was reduced. Results indicated that ABT has enzymes necessary for endogenous production of DHA from EPA and demonstrate that Elovl4b can effectively compensate for absence of Elovl2.es_ES
dc.language.isoenges_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectbluefin tunaes_ES
dc.subjectatún rojoes_ES
dc.subjectThunnus thynnuses_ES
dc.subjectlarval rearinges_ES
dc.subjectBiosynthesises_ES
dc.subjectDocosahexaneonic acides_ES
dc.subjectEicosapentaenoic acides_ES
dc.subjectPolyunsaturated fatty acidses_ES
dc.subjectFish nutritiones_ES
dc.subjectSprecher pathwayes_ES
dc.subjectVery long-chain fatty acidses_ES
dc.titleMolecular and functional characterisation of a putative elovl4 gene and its expression in response to dietary fatty acid profile in Atlantic bluefin tuna (Thunnus thynnus)es_ES
dc.typearticlees_ES
dc.identifier.bibliographicCitationComparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 240. 2019: 110372-110372*
dc.description.versionVersión del editores_ES
dc.publisher.centreCentro Oceanográfico de Murciaes_ES
dc.relation.projectIDNUTRITUNA (AGL2014-52003-C2-1-R)es_ES
dc.rights.accessRightsembargoedAccesses_ES
dc.identifier.doi10.1016/j.cbpb.2019.110372
dc.subject.asfafish es_ES
dc.subject.asfaphysiology es_ES
dc.subject.asfabiochemistry es_ES
dc.subject.asfaaquaculture es_ES
dc.subject.asfaamino acid sequence es_ES


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    Atribución-NoComercial-SinDerivadas 3.0 España
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