Vaccination with UV-inactivated nodavirus partly protects European sea bass against infection, while inducing few changes in immunity
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AuthorsValero, Y. (Yulema); Mokrani, Djamal; Chaves-Pozo, E. (Elena); Arizcun-Arizcun, M. (Marta); Oumouna, M. (Mustapha); Meseguer, J. (José); Esteban, M.A. (M.Ángeles); Cuesta, A. (Alberto)
KeywordsUV, inactivated vaccine, nodavirus, European sea bass, cell-mediated 51 cytotoxicity, immune response
Developing viral vaccines through the ultraviolet (UV) inactivation of virus is promising technique since it is straightforward and economically affordable, while the resulting viruses are capable of eliciting an adequate antiviral immune response. Nodavirus (NNV) is a devastating virus that mainly affects European sea bass juveniles and larvae, causing serious economic losses in Mediterranean aquaculture. In this work, a potential vaccine consisting on UV-inactivated NNV (iNNV) was generated and administered to healthy juveniles of European sea bass to elucidate whether it triggers the immune response and improves their survival upon challenge. First, iNNV failed to replicate in cell cultures and its intraperitoneal administration to sea bass juveniles also failed to produce fish mortality and induction of the type I interferon (IFN) pathway, indicating that the NNV was efficiently inactivated. By contrast, iNNV administration induced significant serum non-specific antimicrobial ...
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