Role of 5 α -dihydrotestosterone in testicular development of gilthead seabream following finasteride administration
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AuthorsGarcía-García, M. (María); Sánchez-Hernández, M. (Miriam); García-Hernández, M.P. (María Pilar); García-Ayala, A. (Alfonsa); Chaves-Pozo, E. (Elena)
Keywords5 α -Reductase, Sex steroids, Steroidogenic enzyme inhibitors, Sparus aurata
In teleosts, spermatogenesis is regulated by pituitary gonadotropins and sex steroids. 5α-dihydrotestosterone (DHT), derived from testosterone (T) through the action of 5α-reductase, has recently been suggested to play a physiologically important role in some fish species. In this study, gilthead seabream, Sparus aurata L., males received an implant of 1μg T/g body mass (bm) or vehicle alone and, 7 days later, 1 mg finasteride (FIN, an inhibitor of 5α-reductase)/kg bm or vehicle. Serum levels of T, 11-ketotestosterone (11KT), DHT and 17β-estradiol (E2), and the mRNA levels of the main enzymes involved in their synthesis, were analysed. T promoted a transient increase in the serum levels of T, 11KT and E2 but a decrease in those of DHT at day 15 following T injection, in accordance with the up-regulation of mRNA levels of the enzymes involved in T transformation to 11KT (coding genes: cyp11b1 and hsd11b) and the down-regulation of mRNA levels of the enzyme responsible for T transformation ...
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