Chromophoric signatures of microbial by-products in the dark ocean

Abstract

Detailed examination of the absorption spectra from dark ocean samples allowed us to identify and deconvolve two distinct chromophores centered to 302 nm (UV) and 415 nm (visible) from the exponential decay curve characteristics of humic substances. The UV chromophore was ubiquitous in intermediate and deep waters, and it has been proposed as the secondary absorption peak of nitrate. The visible chromophore was prominent at the central and intermediate water masses of the North Pacific, and it has been proposed as cytochrome c. Subtraction of the modeled absorption spectra of the two chromophores from the measured absorption spectrum of the samples leads to a spectral slope overestimation by 13.3+-6.0% for S 275-295 and 14.8+-10.6% for S 350-400. To only consider the chromophoric fraction of DOM, the absorption spectra of nitrate should be substracted in samples with a [NO3-]:a 302 ration > 70 micromoles