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dc.contributor.authorStuken, A. (Anke)
dc.contributor.authorRiobó, P. (Pilar) 
dc.contributor.authorFranco, J.M. (José Mariano) 
dc.contributor.authorJakobsen, K.S.
dc.contributor.authorGuillou, L. (Laure)
dc.contributor.authorFigueroa, R.I. (Rosa Isabel) 
dc.date.accessioned2017-09-13T11:38:39Z
dc.date.available2017-09-13T11:38:39Z
dc.date.issued2015-05-01
dc.identifier.urihttp://hdl.handle.net/10508/11151
dc.description.abstractDinoflagellates are microscopic aquatic eukaryotes with huge genomes and an unusual cell regulation. For example, most genes are present in numerous copies and all copies seem to be obligatorily transcribed. The consequence of the gene copy number (CPN) for final protein synthesis is, however, not clear. One such gene is sxtA, the starting gene of paralytic shellfish toxin (PST) synthesis. PSTs are small neurotoxic compounds that can accumulate in the food chain and cause serious poisoning incidences when ingested. They are produced by dinoflagellates of the genera Alexandrium, Gymnodium, and Pyrodinium. Here we investigated if the genomic CPN of sxtA4 is related to PST content in Alexandrium minutum cells. SxtA4 is the 4th domain of the sxtA gene and its presence is essential for PST synthesis in dinoflagellates. We used PST and genome size measurements as well as quantitative PCR to analyze sxtA4 CPN and toxin content in 15 A. minutum strains. Our results show a strong positive correlation between the sxtA4 CPN and the total amount of PST produced in actively growing A. minutum cells. This correlation was independent of the toxin profile produced, as long as the strain contained the genomic domains sxtA1 and sxtA4.es_ES
dc.language.isoenges_ES
dc.subjectdinoflagellateses_ES
dc.subjectAlexandrium minutumes_ES
dc.subjectsaxitoxin (STX)es_ES
dc.subjectparalytic shellfish toxin (PST)es_ES
dc.subjectstxAes_ES
dc.subjectgenome sizees_ES
dc.subjectgene dosagees_ES
dc.titleParalytic shellfish toxin synthesis is related to genomic sxtA4 copy number in Alexandrium minutum strainses_ES
dc.typearticlees_ES
dc.identifier.bibliographicCitationFrontiers in Microbiology, 6. 2015: 404(1)-404(10)*
dc.description.versionPostprintes_ES
dc.publisher.centreCentro Oceanográfico de Vigoes_ES
dc.rights.accessRightsopenAccesses_ES
dc.identifier.doi10.3389/fmicb.2015.00404
dc.subject.asfamicrobiologyes_ES
dc.subject.asfaresearches_ES


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