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dc.contributor.authorTalens-Perales, D. (David)
dc.contributor.authorMartín-Navarro, J. (Julia)
dc.contributor.authorGarrido, D. (Diego) 
dc.contributor.authorAlmansa, E. (Eduardo) 
dc.contributor.authorPolaina, J. (Julio)
dc.date.accessioned2017-05-22T09:51:09Z
dc.date.issued2017
dc.identifier.urihttp://hdl.handle.net/10508/10879
dc.description.abstractArtemia is extensively used in aquaculture to feed early stages of cultured marine species. A problem associated with this practice is that Artemia fails to supply some essential nutrients. As a possible solution, we have devised a procedure to make Artemia a vehicle for exogenous nutrients and other bioactive compounds. It consists of the construction of chimeric proteins composed of a chitin-binding domain, which binds to the cuticle of Artemia, and a carrier domain that conveys a functional property. As confirmatory examples, we describe the successful fixation to Artemia's metanauplii of two hybrid proteins: a β-galactosidase from the thermophilic bacterium Thermotoga maritima and the jellyfish green fluorescent protein (GFP), both linked to the CBM2 chitin-binding domain from the hyperthermophilic archaeon Pyrococcus furiosus. Positive results of experiments carried out ex vivo and in vivo show the validity of this approach. The methodology used could become a general procedure for the attachment of different kinds of bioactive compounds, such as enzymes, hormones, antibiotics, etc., to the cuticle of Artemia as well as other arthropods.es_ES
dc.language.isoenges_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectArthropods cuticlees_ES
dc.subjectβ-galactosidasees_ES
dc.subjectCarbohydrate binding domaines_ES
dc.subjectChimeric proteines_ES
dc.subjectChitines_ES
dc.subjectGFPes_ES
dc.titleFixation of bioactive compounds to the cuticle of Artemiaes_ES
dc.typearticlees_ES
dc.identifier.bibliographicCitationAquaculture (Elsevier), 474. 2017: 95-100*
dc.description.versionEn prensaes_ES
dc.publisher.centreCentro Oceanográfico de Canariases_ES
dc.rights.accessRightsopenAccesses_ES
dc.subject.asfafishes_ES
dc.subject.asfabiomarkerses_ES
dc.subject.asfafixationes_ES


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    Atribución-NoComercial-SinDerivadas 3.0 España
    Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España