Tissue distribution of lipid and fatty acid metabolism and transcription factors genes in adult Atlantic bluefin tuna (Thunnus thynnus L.)
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AuthorsBetancor, M.B.; Ortega-García, A. (Aurelio); de-la-Gándara, F. (Fernando); Tocher, D.R.; Mourente, G.
fatty acid metabolism
transcription factor genes
To determine expression of the major lipid pathways in tissues of adult bluefin tuna 8 individuals were used for collecting samples for tissue expression of key lipid metabolism genes. Triplicate sets of samples of brain, gills, heart, kidney, spleen, liver, intestine, white muscle, red muscle, adipose tissue ovary and testis were collected. Expression of genes was determined by qPCR. Tissue expression profiles showed that PUFA biosynthetic pathway genes were expressed in all tissues examined, highest expression in brain, liver and testis. Elongase elovl5 showed higher expression than desaturase fads2d6 in all other tissues, with low expression of in red muscle and ovaries. Transcription factors, pparα and pparγ showed parallel expression, with adipose tissue with the highest relative copy number, followed by intestine>testis>liver. The expression of lxr was low in liver, with highest expression in testis, brain and kidney. Similarly, rxr was poorly expressed in liver with ...
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